This four-day workshop is ideal for researchers interested in developing neural lineages from iPSCs. Team taught by active researchers from Johns Hopkins, the NIH, the FDA, and Thermo Fisher Scientific, participants will have the opportunity to gain a solid foundation in iPSC methods with emphasis on deriving iPSC and differentiating to different neural lineage. Functional assays will be discussed in lectures for assessing neuronal differentiation. Applications of Neural derived IPSC will be discussed to neuropharmacology, toxicity testing, and therapy.
Lecture and Hands-on Interactive Training
Team taught by active researchers
Comprehensive binder containing workshop material
Space limited to 24 participants
Registration Fee: $995
"As always, Bio-Trac not only met my expectations, but exceeded them! I will absolutely be taking more classes with BioTrac - their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle."
Assistant Professor, The Ohio State University
"By nature it was short and intense, yet it proved comprehensive and satisfying. Provided valuable hands-on lab experiences as well. Highly recommend and I look forward to other course offerings."
Uniformed Services University for the Health Sciences (USUHS)
iPSC: Reprogramming, Differentiation and Gene Editing with CRISPR 8/16
"It was my first experience with professional development workshop in the USA. I liked it a lot. It was well organized, flexible and friendly. It helped to put in order my preceding knowledge and gain more."
Staff Scientist II, Maine Medical Center Research Institute
"This is a very well organized program. Lectures always convey the most advanced knowledge and technologies. Hands-on practice is also very helpful. Highly recommend to colleagues in or out of NIH community."
Research Fellow, NEI/NIH
iPSC: Reprogramming, Differentiation and Gene Editing with CRISPR 8/16
"Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days."
PHD Student, University of Central Florida
"Bio-Trac offers wonderful workshops that provide up-to-date, useful technological information and knowledge important for my research applications."
Thuy Phung, MD, PhD
Baylor College of Medicine
James Kehler VMD, PhD
Overview of Stem Cell Biology
Basics of Cell Reprogramming
Induced Pluripotent Stem Cells:
• Review of Primary Human iPSC Cultures
• Live-staining of Primary iPSC Colonies
• Picking Human iPSC Colonies
• Staining iPSCs for Alkaline Phosphatase Activity
- Feeder-free Media Development
- Non-integrated Human iPSC Generation and Cell Therapy Model of Ischemic Retinopathy
Laboratory: Maintenance of High-quality iPSCs:
• Passaging PSCs in a Feeder-free Culture System
• Grooming Techniques to Remove Spontaneous Differentiation (Jeffrey Fergus, Thermo-Fisher)
Dr. Joseph Bressler is a research scientist at Kennedy Krieger Institute. He is also an associate professor of environmental health sciences at the Bloomberg School of Public Health at Johns Hopkins University.
Dr. Bressler received his bachelor's of science degree in biology from the State University of New York at Stony Brook in 1973 and his doctoral degree in physiology from Rutgers University in 1978. His post-doctoral training at UCLA was in neurosciences, where he studied the involvement of glial cells in response to toxic agents. After his post-doctoral training, Dr. Bressler continued his studies on glial cells at the National Institutes of Health, Bethesda, MD. Dr. Bressler has been a research scientist at the Kennedy Krieger Institute since 1988.
James Kehler VMD, PhD is a comparative stem cell biologist who thrives on developing productive collaborations to translate scientific discoveries into transformative products. He trained at the University of Pennsylvania, where he received his VMD in 2002, and PhD in Cell and Molecular Biology in 2004. James has worked as a visiting researcher at the National Institutes of Health for over 10 years, where he and his collaborators at NEI, NCI, NINDS, NIDDK and NIAAA developed animal and stem cell-based models of human diseases. He has run workshops on reprogramming both at the NIH, as well as internationally. James has worked and consulted for several stem cell companies from product development and management to directing custom reprogramming and gene-editing services. James recently joined MTI-GlobalStem, as Director of Scientific Alliances to foster collaborative research projects with academic, biotech and pharmaceutical partners.
Guest Lecturers and Lab Instructors
|David M. Panchision, PhD
David Panchision is the Chief of the Developmental Neurobiology Program at the National Institute of Mental Health. Dr. Panchision coordinates funding initiatives for the use of induced pluripotent stem cells (iPSCs) to study mental illness. He is Science Officer overseeing several cooperative agreements related to the use of iPSCs, including academic-industry partnerships (PAR-13-225) to use iPSCs to develop validated platforms for identifying novel targets and developing new therapeutics to treat mental illness. Prior to joining NIMH, he was Assistant Professor at Children’s National Medical Center and George Washington University in Washington, DC, where his research focused on the interaction between morphogen and oxygen response signaling in both normal neural stem cells and patient‐derived brain cancer stem cells.
|Donald W. Fink, Jr., PhD
Dr. Donald Fink is in the Cell Therapy Branch, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies (OTAT), Center for Biologics Evaluation and Research (CBER), FDA. He possesses over 20-years of regulatory review experience evaluating applications for a diversity of products including cell-based therapies, recombinant proteins, monoclonal antibody-based reagents, therapeutic vaccines, medical devices used for collection of cellular blood components, cell selection, or preparation of autologous cellular grafts; and combination products.
Presently, Dr. Fink is engaged in regulatory activities pertaining to investigational products comprised of or derived from stem cells. He oversees an extensive portfolio of applications that includes hematopoietic, mesenchymal, cord blood, placenta-derived, and pluripotent stem cell-derived cellular products.
|Ilyas Singec, MD, PhD
Ilyas Singeç joined NCATS in 2015 as the director of Stem Cell Translation Laboratory in the Division of Pre-Clinical Innovation. Singeç translates stem cell discoveries into clinical applications, focusing on the development of new assays (tests), drugs and cell therapies.
Prior to joining NCATS, Singeç carried out postdoctoral work first at the National Institute of Neurological Disorders and Stroke and then at the Sanford Burnham Prebys Medical Discovery Institute in La Jolla, California, where he also served as staff scientist and director of cell reprogramming. Most recently, Singeç worked in the pharmaceutical and entrepreneurial industries.
Singeç earned his M.D. and Ph.D. summa cum laude in Germany at the Universities of Bonn and Freiburg, completing his residency in clinical neuropathology and neuroanatomy in Freiburg.
|Tea Soon Park, PhD
Dr. Park is a Research Associate at Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine. Foundation of her researches is on generation of clinically relevant human induced pluripotent stem cells (iPSC), differentiation of human pluripotent stem cells (hPSC) into hemato-vascular lineage and enhancement of functional pluripotency using naïve reversion method. Using these technologies, her current research focuses on treatment of ischemic damages (e.g. ischemic retinopathy) and diabetic vascular complications with iPSC derived progenitor cells.
FDA Guidelines for Using IPSc in Transplants
In vivo and in vitro iPSC correlates of neurodevelopment
Differentiating iPSCs Neural Induction of PSCs (Part I):
• Harvesting and Plating Cells
• Expanding P0 NSCs
• Cryopreservation of NSCs
• ICC of Neuronal Progenitors (Part I): Blocking and Addition of Primary Antibodies
Differentiation into Dopaminergic Neurons (Part I):
• Harvesting & Plating Cells for Differentiation (Day 0)
• Expansion: Passaging Floor Plate Progenitors (Day 10)
• Maturation: Floor plate sphere dissociation (Day 16) (Jeffrey Fergus, Thermo-Fisher)
- Using iPS Cells for Biological and Therapeutics Discovery: Challenges in Protocols, Assays
- Leveraging the Translation of iPS Cells
Laboratory: Differentiating iPSCs Neural Induction of PSCs (Part II):
• Maintenance of cultures from Thursday.
• ICC of Neuronal Progenitors (Part II): Addition of secondary antibodies & Imaging
Laboratory: Differentiation into Dopaminergic Neurons (Part II):
• Observation of Cultures from Thursday. (Jeffrey Fergus, Thermo-Fisher)