Dec 3-6, 2019

Tuesday - Friday

Germantown, MD

The Bioscience Education Center

9:00am-5:00pm

1 Hour Lunch Break

This course will also be offered on 4/21/20 & 10/27/20.

This hands-on training workshop is ideal for research and translational biology scientists who are looking for a balanced theoretical vs. hands-on introduction to isolation, quantitation, analysis and engineering of exosomes. Topics include: Exosome Isolation; Exosome quantitation; cDNA Library Generation; and Exosome Biomarker Discovery

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Lecture and Hands-on Interactive Training
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Team taught by active researchers
thumbnail drive
Thumbnail drive with Lectures and Workshop material
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Space limited to 20 participants
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Registration Fee: $995

Workshop Sponsor

Course Co-Director

Dr. Mahendra Rao

Dr. Mahendra Rao

Course Co-Director

Dr. James Kehler

Dr. James Kehler

Lectures will include understanding the expanding roles of exosomes in normal physiology and disease processes, as well as the development of new diagnostic tests and potential therapies using exosomes as well as exosome engineering and biomarker discovery.

  • Molecular Mechanisms Involved in the Biogenesis, Release and Uptake of Exosomes and Micro Vesicles and Their Role in Cell to Cell Signaling
  • Effect of Viral Infection on Exosome Production: Lessons from HIV and Other RNA Viruses Controlling Parthenogenesis;
  • Exosome Engineering
  • Exosomal microRNAs: An Introduction to Intracellular Social Media
  • Role of Exosomes in Regenerative Medicine and the Challenges that Remain
  • Exosomes from HTLV-1 and Ebola Infected Cells: Effects on Donor and Recipient Cells
  • Diagnostic use of Exosomes in Disease States/Novel Biomarker Discovery from Analysis of Exosomes from Patient Samples
  • Tracking and Monitoring Extracellular Vesicles in vivo;

Day 1

  • Exosome Isolation: from Serum, from Media;
  • Quantitation: Protein Quantitation by BCA, miRNA;
  • Profiling: Extract RNA from Isolated Serum Exosomes, Bioanalyze Samples

Day 2

  • Harvest Exosomes from Tissue Culture (TC) Samples;
  • TC samples: Protein Quantitation BCA;
  • Characterization:
    • Begin SDS-PAGE Western Blots;
    • miRNA profiling: Extract RNA from Isolated TC Exosomes;
    • cDNA Library Generation;
  • Engineering:
    a. Labeling with ExoGlow Protein & Membrane,
    b. ExoFect Exosomes with Texas-Red siRNA

Day 3

  • Apply Labeled Exosomes to Cells;
  • miRNA profiling: Prepare and Run qPCR,
  • Visualize Exosomes from Engineered Cells

Day 4

  • Quantitation: Begin ELISA-Ultra;
  • Data Review: Western Blot and qPCR Results;
  • Complete ELISA, Review Results

Course Co-Director

Mahendra Rao received his MD (MBBS) from Bombay University in India and his PhD in Developmental Neurobiology from the California Institute of Technology. Mahendra Rao is internationally known for his research involving, IPSC (induced pluripotent stem cells) human embryonic stem cells (hESCs) and other somatic stem cells and has worked in the stem cell field for more than twenty years with stints in academia, government and regulatory affairs and industry. Dr. Rao   has an extensive background teaching medical and graduate students, as well as postdoctoral fellows at institutions including Johns Hopkins University School of Medicine, The National Centre for Biological Sciences in Bangalore, India, and the University of Utah School of Medicine. Dr. Rao has published more than 350 papers on stem cell research and is the co-founder of a neural stem cell company Q therapeutics based in Salt lake City (Utah) and more recently NxCell based in California. Dr. Rao has also served on advisory panels to the governments of the U.S., Singapore and India on hESC and iPSC policy.

Until 2010 Dr. Rao led the Stem Cell and Regnerative Medicine division at LiFE Technologies and also served as the Chair of the CBER (FDA) advisory committee (CTGTAC). Till 2014 Dr. Rao was the founding Director of the NIH Center of Regenerative Medicine and also the Chief of the Laboratory of Stem cell Biology at the NIH.

Dr. Rao is currently on the Board at Q therapeutics and PanCella serves as a consultant of several companies in the regenerative medicine field. Dr. Rao also serves on several scientific advisory boards, journal editorial boards and oversight committees and advisory panels for large scale projects related to stem cell biology. His clients include several stem cell and regenerative medicine companies such Stempeutics, Eyestsem, CBR and Megakaryon. He also contributes his expertise on regulatory affairs by serving on regulatory forums and as the CIRM and ISSCR liaison to the ISCT.

Dr. Rao was recently named one of the top ten influential people in the stem cell field and was honored recently by the Federation of Biologists (FABA) India for his achievements in the stem cell field and awarded the NBRI medal (India) for his contributions to neuroscience research.

Course Co-Director

James Kehler VMD, PhD is a comparative stem cell biologist who thrives on developing productive collaborations to translate scientific discoveries into transformative products. He trained at the University of Pennsylvania, where he received his VMD in 2002, and PhD in Cell and Molecular Biology in 2004. James has worked as a visiting researcher at the National Institutes of Health for over 10 years, where he and his collaborators at NEI, NCI, NINDS, NIDDK and NIAAA developed animal and stem cell-based models of human diseases. He has run workshops on reprogramming both at the NIH, as well as internationally. James has worked and consulted for several stem cell companies from product development and management to directing custom reprogramming and gene-editing services. James recently joined MTI-GlobalStem, as Director of Scientific Alliances to foster collaborative research projects with academic, biotech and pharmaceutical partners.

Senny Nordmeier, PhD
Dr. Nordmeier obtained her PhD in biochemistry from the University of Nevada, Reno. Her graduate studies were on how purinergic mechanisms could influence breast cancer metastasis, which eventually led to the examination of extracellular vesicles as a mediator between tumor cells and the nearby blood vessels. She has a diverse skill set with experiences in microscopy, cancer mouse models, molecular biology, and protein chemistry. As a scientist at SBI, she is leveraging her knowledge in cancer and EV biology to help driving product development efforts with the goal to provide new research tools that can facilitate the advancement of the EV field.
Stephen J. Gould, Ph.D.Stephen J. Gould, PhD
Dr. Gould, PhD is Professor of Biological Chemistry at Johns Hopkins University School of Medicine. A cell biologist and biochemist, Dr. Gould’s laboratory investigates the biogenesis and uptake of exosomes, as well as the formation and infectivity of HIV and other retroviral particles. Dr. Gould is co-director of the Johns Hopkins University Biological Chemistry Graduate Program and serves as the President of the American Society of Exosomes and Microvesicles.
Fatah Kashanchi, PhD
Dr. Kashanchi obtained his Ph.D. from the University of Kansas with Dr. Charles Wood who was instrumental in the development of the Abbot HIV-1 gp120 ELISA assay in 1986 and was trained under supervision of Dr. Susumu Tonegawa (Nobel Laurate for Medicine, 1987) at MIT. Dr. Kashanchi further trained in Dr. John Brady’s lab at NIH/NCI from 1991-2000 and published more than 30 papers during his postdoc and senior investigator Tenure. He then moved to George Washington Medical School in Washington DC where he ran his lab for 8 years. Finally he moved to George Mason University in 2011 and became the director or research on Biodefense related organisms. He is currently head of the Laboratory of Molecular Virology (LMV) at GMU. He has obtained more than $15.8M in funding (NIH, DOD, DOE, and Keck) since his departure from NIH in 2000. He has published 201 peer-reviewed manuscripts (h index = 53), and has served as an editorial board and reviewer for Retrovirology, JBC, J. Virol, Virology, NAR, 4 PLoS Journals, Cell, Molecular Cell, Nature, Medicine, and Science Translational Medicine. He is also a regular NIH study section member and has served on 141 panels since 2000. He has chaired 23 of these study sections.  
Sukbir Kaur, PhD
Dr. Kaur obtained her Ph.D.  from Guru Nanak Dev University (Punjab, India) and her post-doctoral fellowship training at  Genome Technology branch (NHGR1) where she developed her interest in miRNAs and non-coding RNAs. Later, she became a staff scientist in Biochemical Pathology Section , where she expertise  her research in  area of  extracellular vesicles (Exosomes). Her research have found that donor  exosomes can modulate functional role of recipient cells via releasing their internal contents which also carry coding and noncoding RNAs.  Her research interests are to explore cell-cell communications via Exosomes and their functional role in cancer and normal stem cell biology. Her research work has been recognized in the extracellular vesicle field by being a frequent invited speaker at their national and international meetings, and she is considered to be a leader in this area. She has received many awards and co-author of more than 30 scientific publications and 2 patents in her research field. She serves on peer review panels of the PLoS One, Scientific Reports and JOVE.
Samarjit (Sam) Das, PhD
Dr. Das is an Assistant Professor in the Department of Anesthesiology and Critical Care Medicine and the Department of Pathology at Johns Hopkins School of Medicine. His laboratory investigates the functional aspects of microRNAs in the pathophysiology of various disease conditions, including cardiovascular disease. Recently, the Das laboratory is focusing on cell-cell communication with microRNA in disease conditions. One of the major findings from their recent work is that exosomal miRNA transfer from one tissue to another can cause a disease phenotype. Dr. Das is a founding member of the Program for microRNA Biology at Johns Hopkins and serves as the Chair of the Early Career Investigator section of the International Society of Heart Research.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876