April 9-12, 2019

Tuesday - Friday

Germantown, MD

The Bioscience Education Center

9:00am-5:00pm

1 Hour Lunch Break

Offered again April 9-12, 2019

* NEW OFFERING * This hands-on training workshop is ideal for research and translational biology scientists who are looking for a balanced theoretical vs. hands-on introduction to isolation, quantitation, analysis and engineering of exosomes. Topics include: Exosome Isolation; Exosome quantitation; cDNA Library Generation; and Exosome Biomarker Discovery

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Lecture and Hands-on Interactive Training
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Team taught by active researchers
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Comprehensive binder containing workshop material
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Space limited to 20 participants
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Registration Fee: $995

Workshop Sponsor

Course Director

Dr. James Kehler

Dr. James Kehler

Exosomes Lecture Topics:

Lectures will include understanding the expanding roles of exosomes in normal physiology and disease processes, as well as the development of new diagnostic tests and potential therapies using exosomes as well as exosome engineering and biomarker discovery.

Day 1

  • Exosome Isolation: from Serum, from Media;
  • Quantitation: Protein quantitation by BCA, miRNA
  • Profiling: Extract RNA from Isolated Serum Exosomes, Bioanalyze Samples

Day 2

  • Harvest Exosomes from Tissue Culture (TC) Samples;
  • TC samples: Protein Quantitation BCA;
  • Characterization: Begin SDS-PAGE Western Blots;
  • miRNA profiling: Extract RNA from Isolated TC Exosomes;
  • Prepare cDNA and Anneal Adapters;
  • cDNA Library Generation; Engineering: a. Labeling with ExoGlow Protein & Membrane, b. ExoFect Exosomes with Texas-Red siRNA

Day 3

  • Apply Labeled Exosomes to Cells;
  • miRNA profiling: Prepare and Run qPCR, Visualize Exosomes from Engineered Cells

Day 4

  • Quantitation: Begin ELISA-Ultra;
  • Data Review: Western Blot and qPCR Results;
  • Complete ELISA, Review Results

Course Director

James Kehler VMD, PhD is a comparative stem cell biologist who thrives on developing productive collaborations to translate scientific discoveries into transformative products. He trained at the University of Pennsylvania, where he received his VMD in 2002, and PhD in Cell and Molecular Biology in 2004. James has worked as a visiting researcher at the National Institutes of Health for over 10 years, where he and his collaborators at NEI, NCI, NINDS, NIDDK and NIAAA developed animal and stem cell-based models of human diseases. He has run workshops on reprogramming both at the NIH, as well as internationally. James has worked and consulted for several stem cell companies from product development and management to directing custom reprogramming and gene-editing services. James recently joined MTI-GlobalStem, as Director of Scientific Alliances to foster collaborative research projects with academic, biotech and pharmaceutical partners.

Tomer Cooks, Ph.D Tomer Cooks, PhD
Tomer Cooks, PhD is a tumor cell biologist by training, having spent three years at the Weizmann Institute of Science in Israel as a post-doctoral fellow then moving to the NIH National Cancer Institute in 2013. As a visiting fellow at the NCI, Tomer entered the world of extracellular vesicles and studied cell-to-cell interactions in the context of the tumor microenvironment. He has organized and moderated both intramural and extramural NIH workshops on extracellular vesicles. His works have been presented in several international meetings and has received several awards.
Stephen J. Gould, Ph.D.Stephen J. Gould, PhD
Stephen J. Gould, Ph.D., is Professor of Biological Chemistry at Johns Hopkins University School of Medicine. A cell biologist and biochemist, Dr. Gould’s laboratory investigates the biogenesis and uptake of exosomes, as well as the formation and infectivity of HIV and other retroviral particles. Dr. Gould is co-director of the Johns Hopkins University Biological Chemistry Graduate Program and serves as the President of the American Society of Exosomes and Microvesicles.
Dimitrios Kapogiannis, PhD
Dimitrios Kapogiannis is a Clinical Investigator at the Laboratory of Neurosciences at the National Institute on Aging (NIA) and adjunct Associate Professor at the Department of Neurology at Johns Hopkins University. He earned his Medical Degree from the National University of Athens, Greece, and completed his Neurology residency training at Harvard University/Massachusetts General /Brigham and Women’s Hospitals, followed by a clinical fellowship at the National Institute of Neurological Disorders and Stroke. He is ABPN-certified in Neurology and UCNS-certified in Behavioral Neurology. Dr. Kapogiannis leads a Lab at the National Institute on Aging conducting translational and clinical studies on various neurodegenerative diseases, with a primary focus on biomarkers and therapeutics of Alzheimer’s disease. He has been a pioneer in deriving exosomes enriched for neuronal and astrocytic origin from blood and using them as a source of biomarkers for neurologic diseases.
Fatah Kashanchi, Professor; PhD
Dr. Kashanchi obtained his Ph.D. from the University of Kansas with Dr. Charles Wood who was instrumental in the development of the Abbot HIV-1 gp120 ELISA assay in 1986 and was trained under supervision of Dr. Susumu Tonegawa (Nobel Laurate for Medicine, 1987) at MIT. Dr. Kashanchi further trained in Dr. John Brady’s lab at NIH/NCI from 1991-2000 and published more than 30 papers during his postdoc and senior investigator Tenure. He then moved to George Washington Medical School in Washington DC where he ran his lab for 8 years. Finally he moved to George Mason University in 2011 and became the director or research on Biodefense related organisms. He is currently head of the Laboratory of Molecular Virology (LMV) at GMU. He has obtained more than $15.8M in funding (NIH, DOD, DOE, and Keck) since his departure from NIH in 2000. He has published 201 peer-reviewed manuscripts (h index = 53), and has served as an editorial board and reviewer for Retrovirology, JBC, J. Virol, Virology, NAR, 4 PLoS Journals, Cell, Molecular Cell, Nature, Medicine, and Science Translational Medicine. He is also a regular NIH study section member and has served on 141 panels since 2000. He has chaired 23 of these study sections.  
Elena Miñones-Moyano, PhD
Dr. Miñones-Moyano is a Technical Specialist Scientist at System Biosciences (SBI). She completed her PhD in Human Genetics at the Centre for Genomic Regulation (Barcelona, Spain), and subsequently worked as a Postdoctoral Scientist in the Human Genetics Unit of the Medical Research Council in Edinburgh (UK) and in the Neurology Department at UCSF (USA). Throughout her over ten years of track-record academic research, she investigated the contribution of small non-coding RNA deregulation to the pathogenesis of neurodegenerative diseases, such as Parkinson, Huntington and Alzheimer; as well the mechanisms triggering the deregulation. Her continued interest in new technologies and her passion for helping Scientists to move their research forward, led her to join SBI where she where she now manages the training and outreach programs, and provides technical support and experimental design guidelines with a strong focus on extracellular vesicle research and biomarker discovery.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876