May 8-11, 2018

Tuesday - Friday

Germantown, MD

The Bioscience Education Center

9:00am-5:00pm

1 Hour Lunch Break

* NEW OFFERING *

This hands-on training workshop is ideal for research and translational biology scientists who are looking for a balanced theoretical vs. hands-on introduction to isolation, quantitation, analysis and engineering of exosomes. Topics include: Exosome Isolation; Exosome quantitation; cDNA Library Generation; and Exosome Biomarker Discovery

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Lecture and Hands-on Interactive Training
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Team taught by active researchers
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Comprehensive binder containing workshop material
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Space limited to 20 participants
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Registration Fee: $995

Workshop Sponsor

Course Director

Dr. James Kehler

Dr. James Kehler

Exosomes Lecture Topics:

Lectures will include understanding the expanding roles of exosomes in normal physiology and disease processes, as well as the development of new diagnostic tests and potential therapies using exosomes as well as exosome engineering and biomarker discovery.

Day 1

  • Exosome Isolation: from Serum, from Media;
  • Quantitation: Protein quantitation by BCA, miRNA
  • Profiling: Extract RNA from Isolated Serum Exosomes, Bioanalyze Samples

Day 2

  • Harvest Exosomes from Tissue Culture (TC) Samples;
  • TC samples: Protein Quantitation BCA;
  • Characterization: Begin SDS-PAGE Western Blots;
  • miRNA profiling: Extract RNA from Isolated TC Exosomes;
  • Prepare cDNA and Anneal Adapters;
  • cDNA Library Generation; Engineering: a. Labeling with ExoGlow Protein & Membrane, b. ExoFect Exosomes with Texas-Red siRNA

Day 3

  • Apply Labeled Exosomes to Cells;
  • miRNA profiling: Prepare and Run qPCR, Visualize Exosomes from Engineered Cells

Day 4

  • Quantitation: Begin ELISA-Ultra;
  • Data Review: Western Blot and qPCR Results;
  • Complete ELISA, Review Results

Course Director

James Kehler VMD, PhD is a comparative stem cell biologist who thrives on developing productive collaborations to translate scientific discoveries into transformative products. He trained at the University of Pennsylvania, where he received his VMD in 2002, and PhD in Cell and Molecular Biology in 2004. James has worked as a visiting researcher at the National Institutes of Health for over 10 years, where he and his collaborators at NEI, NCI, NINDS, NIDDK and NIAAA developed animal and stem cell-based models of human diseases. He has run workshops on reprogramming both at the NIH, as well as internationally. James has worked and consulted for several stem cell companies from product development and management to directing custom reprogramming and gene-editing services. James recently joined MTI-GlobalStem, as Director of Scientific Alliances to foster collaborative research projects with academic, biotech and pharmaceutical partners.

Stephen J. Gould, Ph.D.Stephen J. Gould, Ph.D.
Stephen J. Gould, Ph.D., is Professor of Biological Chemistry at Johns Hopkins University School of Medicine. A cell biologist and biochemist, Dr. Gould’s laboratory investigates the biogenesis and uptake of exosomes, as well as the formation and infectivity of HIV and other retroviral particles. Dr. Gould is co-director of the Johns Hopkins University Biological Chemistry Graduate Program and serves as the President of the American Society of Exosomes and Microvesicles.
Fatah Kashanchi, Professor; Ph.D.
Dr. Kashanchi obtained his Ph.D. from the University of Kansas with Dr. Charles Wood who was instrumental in the development of the Abbot HIV-1 gp120 ELISA assay in 1986 and was trained under supervision of Dr. Susumu Tonegawa (Nobel Laurate for Medicine, 1987) at MIT. Dr. Kashanchi further trained in Dr. John Brady’s lab at NIH/NCI from 1991-2000 and published more than 30 papers during his postdoc and senior investigator Tenure. He then moved to George Washington Medical School in Washington DC where he ran his lab for 8 years. Finally he moved to George Mason University in 2011 and became the director or research on Biodefense related organisms. He is currently head of the Laboratory of Molecular Virology (LMV) at GMU. He has obtained more than $15.8M in funding (NIH, DOD, DOE, and Keck) since his departure from NIH in 2000. He has published 201 peer-reviewed manuscripts (h index = 53), and has served as an editorial board and reviewer for Retrovirology, JBC, J. Virol, Virology, NAR, 4 PLoS Journals, Cell, Molecular Cell, Nature, Medicine, and Science Translational Medicine. He is also a regular NIH study section member and has served on 141 panels since 2000. He has chaired 23 of these study sections.  
Archana Gupta, PhD
Dr. Gupta is a senior scientist at System Biosciences (SBI) with research expertise in the field of extracellular vesicles and viral infections. She received her BSc (Hons) in Biotechnology from Northumbria University, United Kingdom and PhD in Immunology and Microbiology from Drexel University College of Medicine, USA. As part of her graduate work, she investigated the role of monocyte-derived exosomes in mediating neurocognitive impairment in HIV/HCV-coinfected subjects. Her continued passion and interest in the area of extracellular vesicles led her to join SBI where she now develops novel tools and technologies for basic and preclinical exosome research.
Tomer Cooks, Ph.D Tomer Cooks, Ph.D
Tomer Cooks, Ph.D is a tumor cell biologist by training, having spent three years at the Weizmann Institute of Science in Israel as a post-doctoral fellow then moving to the NIH National Cancer Institute in 2013. As a visiting fellow at the NCI, Tomer entered the world of extracellular vesicles and studied cell-to-cell interactions in the context of the tumor microenvironment. He has organized and moderated both intramural and extramural NIH workshops on extracellular vesicles. His works have been presented in several international meetings and has received several awards.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876