March 6-9, 2018

Tuesday - Friday

Germantown, MD

The Bioscience Education Center


1 Hour Lunch Break


This four-day workshop is ideal for researchers interested in developing hepatocytes from iPSCs. Team taught by active researchers, participants will have the opportunity to gain a solid foundation in iPSC methods with emphasis on deriving iPSC and differentiating to hepatocytes. Functional assays will be discussed in lectures for assessing hepatocyte differentiation, drug metabolism, and toxicity testing.

Class size smaller then usual.  Don't miss out, register today!

Lecture and Hands-on Interactive Training
Team taught by active researchers
Comprehensive binder containing workshop material
Space limited to 20 participants
Registration Fee: $995

Workshop Sponsors

Course Director

Course Director Photo

Joseph Bressler, Ph.D.
Research Scientist, Kennedy Krieger Institute

Hepatocyte cultures are used in pharmacology and regulatory toxicology to provide information on drug metabolism and toxicity.  Models using rat hepatocytes have provided inaccurate information because of species differences.   Human hepatocyte cultures from human liver are currently being used but have disadvantages.  For example, cultures form human liver cannot be regenerated, they have limited life span in culture, and there is potential genetic heterogeneity because batches of human hepatocytes come from different donors. Hepatocyte cultures derived from IPSc (HC-iPSc)) express many of the properties displayed in human liver.  HC-iPSc are renewable and could be generated from different genetic backgrounds.  Consequently, HC-iPSc have great utility in pharmacology and regulator toxicology.

  • Introduction to iPSc
  • Methods for inducing pluripotency
  • Biology of hepatocytes
  • Hepatocytes for drug metabolism
  • Conditions for differentiating hepatocytes
  • Models to study hepatocyte injury in vivo and in vitro
  • Hepatocyte cultures in toxicology
  • Hepatocyte culture models for studying infectious diseases
  • iPSc cultures (derivation and maintenance)
  • Hepatocyte differentiation protocol (media, matrix, atmosphere)
  • Cryopreservation
  • Validation methods
  • Conduct a Cyp assay
  • Cytoxicity assays

Course Director

Dr. Joseph Bressler is a research scientist at Kennedy Krieger Institute. He is also an associate professor of environmental health sciences at the Bloomberg School of Public Health at Johns Hopkins University.
Dr. Bressler received his bachelor's of science degree in biology from the State University of New York at Stony Brook in 1973 and his doctoral degree in physiology from Rutgers University in 1978. His post-doctoral training at UCLA was in neurosciences, where he studied the involvement of glial cells in response to toxic agents. After his post-doctoral training, Dr. Bressler continued his studies on glial cells at the National Institutes of Health, Bethesda, MD. Dr. Bressler has been a research scientist at the Kennedy Krieger Institute since 1988.

Guest Lecturers and Lab Instructors

Annika Asplund, Ph.D.
Annika Asplund is a Senior Research Scientist at the Stem Cell Department at Takara Bio Europé in Sweden. She completed her Ph.D. in molecular medicine within the field of cardiovascular prevention at Gothenburg University, Sweden. In 2012 she joined the Takara group with focus on hepatocyte differentiation and maturation from human pluripotent stem cells. Dr. Asplund has extensive experience in protocol development regarding hepatocyte differentiation and characterization as well as dissociation and cryopreservation.
Ingrid Rydstrom
Ingrid Rydström has more than ten years of experience within the field of human pluripotent stem (hPS) cells. Ingrid has extensive experience ranging from e.g. reprogramming of somatic cells to iPS cells, characterization of hPS cells, and toxicity testing using hESC to how to direct the differentiation into e.g. hepatocytes. Today, Ingrid works as Product Manager at Takara Bio Europe in Sweden. Ingrid holds a Master of Science degree in Biotechnology from Chalmers University of Technology.
Lucie Low, Ph.D.
Dr. Lucie Low joined NCATS in 2016 as the Tissue Chip Program Manager for the NIH Microphysiological Systems program, coordinating over 20 research teams working on the development, validation and application of microphysiological systems. Prior to this, she was a Research Fellow at NIH, and Postdoctoral Fellow in Montreal at McGill University, where she studied the effects of chronic pain on brain structure and function. She gained her Bachelors’ Degree from Oxford University (UK) and completed her Masters and PhD in Neuroscience at University College London (UK).
Instructor PhotoTea Soon Park, Ph.D.
Dr. Park is a Research Associate at Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine. Foundation of her researches is on generation of clinically relevant human induced pluripotent stem cells (iPSC), differentiation of human pluripotent stem cells (hPSC) into hemato-vascular lineage and enhancement of functional pluripotency using naïve reversion method. Using these technologies, her current research focuses on treatment of ischemic damages (e.g. ischemic retinopathy) and diabetic vascular complications with iPSC derived progenitor cells.
Jennifer L. Groebner
Jennifer Groebner is currently a Ph.D. candidate at The Catholic University of America in Washington, DC. She currently investigates the molecular mechanisms that lead to the progression of alcoholic liver disease. Her focus is on alcohol-induced changes in microtubule dynamics that lead to early stages of liver injury. Previously, as a Research Microbiologist at the United States Army Medical Research Institute of Infectious Disease (USAMRIID), she developed and evaluated vaccines against viral pathogens (Ebola, Marburg), toxins (botulinum neurotoxin) and bacterial (anthrax, plague) diseases. Jennifer holds a MS in Biotechnology with a bioinformatics concentration from Johns Hopkins University and a BA in Biochemistry from Minnesota State University, Mankato.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876