Bio-Trac’s Thursday Seminar Series – Special Topics in Biotechnology
Bisulfite Pyrosequencing: Quantitative Approach for Assessing DNA Methylation
There continues to be much interest in epigenetics as one of the primary mechanisms that underlie numerous physiological and pathological processes, as well how organisms interact with the environment. In this webinar, I will cover the basics of bisulfite pyrosequencing, which is a highly quantitative method for measuring DNA methylation. I will also provide a brief overview of DNA methylation and use examples from my work to demonstrate how pyrosequencing can be applied in biomedical research.
Modeling the prostate tumor microenvironment using organ-on-a-chip technology
Many of the current two-dimensional (2D) cell culture models used in prostate cancer research fail to recapitulate the prostate tumor microenvironment leading to low predictivity of the models. Physiologically based, 3D in vitro systems offer the potential to enhance the complexity and fidelity of 2D systems as they incorporate many components present in the in vivo tumor including extracellular matrix, stromal cell types, and perfusion. This presentation will review the setup and applications of a prostate tumor-on-a-chip model.
Dimensions of Validation in Multiplex IF: Staining, Imaging, and Image Analysis
The democratization of multiplex IF has resulted in the proliferation of panels and technologies of varying robustness and reproducibility. Variability can stem from staining, imaging, and image analysis. The manner in which a panel is developed and the degree to which is should be validated depend on the intended application. In this webinar, Michael Surace will describe various considerations and best practices for the development and validation of multiplex IF workflow for research and clinical applications.
Sample Processing Considerations for Single Cell Sequencing – A Crucial Component of Experimental Design and Data Interpretation
A successful single cell study starts with a thoughtful consideration of how to prepare a cell or nuclei suspension. Choices on how to prepare sample may be intricately related to tissue type, study design and the experimental questions sought after. Knowledge of how samples were processed, including any enrichment or selection can influence data interpretation in important ways.
Useful Tips on Designing Successful CRISPR Experiments
This presentation will summarize the main molecular features and key applications of CRISPR/Cas9 genome editing technology. Representative examples of CRISPR experimental strategies will be discussed and some practical advices shared on how to design efficient CRISPR systems to achieve gene disruption or more subtle targeted alterations and insertions of desired DNA sequences.
R - Harnessing the Power of the Tidyverse
This walk through presentation will demonstrate the ability to generate multiple plots with a few simple commands.
Separating Viruses from Exosomes
Dr. Kashanchi will discuss the separation of virus away from exosomes using multiple models of virus infection including HIV-1. Functional analysis of the exosomes will be discussed on recipient uninfected cells along with mechanisms of how these exosomes can control the immune cells by either cell death or reprogramming. Finally, he will discuss the latest findings on how to separate Corona viruses away from exosomes since they have similar sizes coming out of infected cells. This will aid in better understanding of the cytokine storm observed in patients post infection.
Rare Cell Detection by Flow Cytometry: Enables in-depth Characterization of Functional Lymphocyte Subsets
This presentation will describe the importance of sample size calculation for flow cytometric analysis and limitations of conventional flow cytometry regarding detection threshold and sensitivity. We will also discuss methods enabling rare cell detection and characterization of low frequency cell populations.