2021 Offerings: 3/22/21 & 11/15/21
*New Offering* This live remote in-depth lecture, laboratory demonstration, protocol review, in silico exercises and discussion is ideal for those research and bench scientists who are interested in a comprehensive introduction to single cell RNA-Seq. The core of this workshop is composed of highlighting key aspects of NSG and RNA-seq methodologies, with subsequent introduction into the modern armamentarium of tools to conduct these experiments. Further emphasis will be placed on such important aspects as sample preparation, quality control validation and enrichment as well as extensive use of different single cell RNA-Seq data analysis tools (Seurat, Monocle, Pseudo-time Analysis, Clustering Analysis in-depth: t-SNE and Principal Component Analysis).
Real-Time Interactive Lecture, Laboratory Protocol and Discussion
Team taught by active researchers
Lectures, Lab Protocols, Reference Materials included
Space limited to 24 participants
No Travel, Convenient and Cost Effective
"Very informative workshop with a very knowledgable lecturer. Covered the various aspects of NGS and provided guidance I needed."
University of the West Indies (Cave Hill)
"It was my first experience with professional development workshop in the USA. I liked it a lot. It was well organized, flexible and friendly. It helped to put in order my preceding knowledge and gain more."
Staff Scientist II
Maine Medical Center Research Institute
"This was a great workshop to become acquainted with Next Generation Sequencing Technologies and applications. I would highly recommend this course to anyone interested in an in-depth hands-on course covering relevant NGS bioinformatics and command line tools. Great experience!"
Henry M. Jackson Foundation
for the Advancement of Military Medicine
"Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days."
University of Central Florida
- Introduction to Next Generation Sequencing (NGS) and RNA-seq
- Single cell RNA-seq: Principles and Methods
- Sample Preparation and Validation for Single Cell Sequencing
- Drop-seq, High Throughput Single Cell RNA-seq
- 10x Genomics: Chromium Controller Single Cell 3 Work Flow
- Illumina-Biorad’s ddSeq: Data Analysis with BaseSpace; SureCell RNA Single-Cell app
- Comparison of Single Cell RNA-Seq Platforms: Drop-Seq, 10X Genomics, Illumina ddSeq and Fluidigm
- Pre-requisites for Single Cell RNA-seq Analysis: Linux command-line & R Review
Lecture and Hands-on In Silica laboratory:
- Single Cell RNA-seq Analysis: Analysis with Seurat; Analysis with Monocle
- Recent Development of Various seq’s: Related Single Cell RNA-seq, SPLiT-seq, Seq-Well
- Differential Gene Expression Analysis for Single Cell RNA-seq
- TSCAN: Pseudo-time Analysis
- Normalizations in Single Cell RNA-seq
- Clustering Analysis in-depth, Particularly for t-SNE and Principal Component Analysis
Wet Laboratory Demonstration, Protocols and Discussion
- Sample Preparation and Validation for Single Cell Sequencing: Tissue Dissociation, Dead Cell Removal as well as other Contaminants, Pre-enrichment Prior to Single-cell Sequencing
Dr. Yun obtained his Ph.D. in computational biology from Boston University, with his research focusing on the aggregation of amyloid beta protein in Alzheimer's disease. Sijung took a postdoc position at the NIH, with the National Cancer Institute (NCI) studying structural bioinformatics and proteomics. Later, he worked at the genomics core in National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). Currently, he is an independent contract bioinformatician primarily working for National Institutes of Health (NIH), C.E.O. of Yotta Biomed, LLC and is a lead instructor in numerous bioinformatics next generation sequencing (NGS) training activities. Dr. Yun had directed our Bio-Trac NGS related workshops since 2009 and has provide NGS instruction to over 700 Bio-Trac participants.
|Michael Kelly, Ph.D.
Dr. Kelly is a Senior Scientist with Frederick National Laboratory who leads the newly established NCI Single Cell Analysis Facility. He was previously a post-doctoral fellow at NIH-NIDCD, where he applied single cell RNA-Seq to study the developmental transcriptional programs of mammalian sensory systems. He has experience with many of the single cell transcriptional profiling methods and platforms and works to stay abreast of advancement in single cell sequencing methods and analysis.
|Eugen C. Buehler, Ph.D.
Eugen Buehler began his work on RNA interference (RNAi) design and the interpretation of RNAi screening results as a member of the Applied Computer Science and Mathematics Department at Merck. He has continued this research since joining NCATS in 2012. He has developed methods for detecting and visualizing RNAi off-target effects, inferring off-targeted transcripts from large-scale screens, and establishing bench-level controls for small interfering RNA experiments. To test and refine these techniques, he collaborates with researchers from NIH, academia and industry. Buehler is a frequently invited speaker at RNAi conferences and other venues. He holds a Ph.D. in computer science from the University of Pennsylvania and a B.S. in physics from North Carolina State University.
|Allissa Dillman, Ph.D.
Dr. Allissa Dillman received her Ph.D. in neuroscience from the graduate partnership program between NIH and the Karolinska Institutet. She studied gene expression regulation, splicing and RNA editing in development and disease. Her specific interest in RNA modifications include base pair mapping in the transcriptome and their potential biological function. In 2015, she joined the National Cancer Institute for her postdoctoral work where she studies epigenetic regulation of splicing and the identification and biological function of novel RNA modifications. Throughout her scientific career she has contributed to more than 30 scientific publications and participates in mentorship and teaching of both bioinformatics and RNA biology. Dr. Dillman has also participated as a team lead for RNA related projects in multiple hackathons at the NIH.
|Niraj Trivedi, Ph.D.
Dr. Trivedi is a branch statistician for Columbus Technologies as a contractor for the Social Behavioral Research Branch at the National Human Genome Research Institute (NHGRI) at the National Institutes of Health. Dr. Trivedi provides statistical, computational, and bioinformatic support to scientists of the institute for their data analysis needs. This includes microarray analysis, statistical analysis, and general analysis in biomedical "big data"