Offered again 12/01/20 - 12/04/20
This four day live remote program is ideal for those who are looking for an in-depth introduction to Flow Cytometry. The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design. Attendees will have laboratory demonstration, protocol reviews and discussion on three different flow platforms (Becton Dickinson, Miltenyi and Bio-Rad) as well as exposure to four software analysis programs.
Real-Time Interactive Lecture, Laboratory Protocol and Discussion
Team taught by active researchers
Lectures, Lab Protocols, Reference Materials included
Space limited to 24 participants
No Travel, Convenient and Cost Effective
" I thoroughly enjoyed this comprehensive and practical presentation of Flow Cytometry – Went back to my home lab and hit the ground running. Thanks for the top-quality training, Bio-Trac!"
FDA NCTR DSB BAMB
" This was a great course. The instructors were very knowledgable & helpful. Without introducing any bias towards particular commercial vendors, the course managed to provide a well balanced flavor for what is out there in terms of options for flow machines and software. The hands on workshops were also very helpful with truly individualized supervision during the classes."
University of Pennsylvania
" Bio-Trac courses are the sure way for one to jump start on a technique’s knowledge and applications base directly from experts and practitioners in the field. They surely give you insights that are not available elsewhere but from years of experiences. knowledge doesn’t stop when a training ends but rather a beginning of one’s development."
Neil H Tan Gana
Ateneo de Manila University, Philippines
"As always, Bio-Trac not only met my expectations, but exceeded them! I will absolutely be taking more classes with BioTrac - their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle."
Assistant Professor, The Ohio State University
"Excellent workshop provided basic and correct gating strategy and respective controls for the specific experiments. Very helpful and whole team was very supportive and explained all my queries at length"
Johns Hopkins Medical Campus (ACCM)
Basic Analytical Techniques, Principles, and Trouble Shooting; Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation); Flow Sorting: Instrument Setup; Parameters; Purification and Labeling of Antibodies for Flow Cytometric and other Applications; Functional Assays for the Flow Cytometer; Multicolor Flow Cytometry and Panel Development; Phenotyping and Analyzing Cell Populations by Flow Cytometry - How to Read Flow Data
Laboratory Demonstration, Protocol Review:
Preparation of Samples for Multicolor Analysis Flow Sorting; Multicolor Analysis, Compensation and Flow Sorting
a. BD Accuri C6:
Staining of Human Peripheral Blood Mononuclear Cells (PBMC) (hands on for each participant) with Basic Flow Panel (4 colors). Flow Cytometer Setup (BD Accuri; two lasers) with Special Emphasis on Compensation. Basics on Flow Cytometer Settings - Voltages, Gating, Dump Channels, Exclusion, and Compensation.
b. Miltenyi MACSQuant Analyzer 10
Advanced Flow Cytometry Laboratory
Analyzing human whole blood and human serum. This rotation includes: Automatic instrument calibration, automatic 8 color compensation, acquisition of whole blood with no RBC lysis, automatic gating/analysis of 8 color immunophenotyping panel, introduction to cytokine multiplex assay, acquisition of cytokine multiplex assay, automatic gating/analysis of cytokine multiplex assay, and automatic shutdown of instrument. In addition to immunophenotyping and multiplex assay, rare cell enrichment and cell counting will be discussed.
c. Bio-Rad S3e Flow Sorter
Emphasis on Sorting Conditions (parameters, factors, strategies) as well as the Specific Challenges Related to Cell Preparation, Efficiency, Recovery, and Alternate Techniques for Cell Population Identification.
d. Sony SH800 Flow Sorter
Single Cell Deposition
In Silico Software Training
a. Introduction to FlowJo Cytometry Analysis Software
b. Introduction to Cyflogic Cytometry Analysis Software; Flow Cytometry - Acquisition/Trouble Shooting/ Software
c. Introduction to FlowLogic Cytometry Analysis Software
d. Introduction to FCS Express Cytometry Analysis Software
Elke Bergmann-Leitner, Ph.D.
Dr. Elke Bergmann-Leitner is the Chief of the Flow Cytometry Center (Malaria Vaccine Branch) at the Walter Reed Army Institute of Research in Silver Spring, MD. She is an adjunct Research Associate Professor of Medicine at the Uniformed Services University of Health Sciences and has been teaching flow cytometry for 25 years. Currently, her main research focus is the development of readout methods capable of identifying immune correlates of protection against infectious diseases.
Wolfgang Leitner, Ph.D.
Dr. Leitner is the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH/NIAID). He received his flow cytometry training at the University of Salzburg where he obtained a MSc in biochemistry and a PhD in Immunology. He has been teaching flow cytometry for 25 years. Before joining NIAID, he worked on melanoma and lymphoma vaccines at NCI/NIH, and at WRAIR as a vaccine consultant.