Dec 1-4, 2020

Tuesday - Friday

9:00am-5:00pm

1 Hour Lunch Break

$995

Workshop Fee

Three 2021 Offerings  2/23/21, 8/23/21, 11/15/21

This four day live remote program is ideal for those who are looking for an in-depth introduction to Flow Cytometry.  The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.  Attendees will have laboratory demonstration, protocol reviews and discussion on three different flow platforms (Becton Dickinson, Miltenyi and Bio-Rad) as well as exposure to four software analysis programs.

Flow Cytometry data analysis software:   FlowJo   I   FlowLogic    I    Cyflogic   I  FCS Express 6

remote learning
Real-Time Interactive Lecture, Laboratory Protocol and Discussion
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Team taught by active researchers
Reference Materials Included
Lectures, Lab Protocols, Reference Materials included
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Space limited to 24 participants
Save Money and Time
No Travel, Convenient and Cost Effective

Course Director

Elke Bergmann-Leitner, Ph.D.
Chief, Flow Cytometry Center
USMMRP/WRAIR

Lectures:

Basic Analytical Techniques, Principles, and Trouble Shooting; Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation); Flow Sorting: Instrument Setup; Parameters; Purification and Labeling of Antibodies for Flow Cytometric and other Applications; Functional Assays for the Flow Cytometer; Multicolor Flow Cytometry and Panel Development; Phenotyping and Analyzing Cell Populations by Flow Cytometry - How to Read Flow Data

Laboratory Demonstration, Protocol Review:

Preparation of Samples for Multicolor Analysis Flow Sorting; Multicolor Analysis, Compensation and Flow Sorting

Laboratory Demonstration:
a.   BD Accuri C6:

Staining of Human Peripheral Blood Mononuclear Cells (PBMC) (hands on for each participant) with Basic Flow Panel (4 colors). Flow Cytometer Setup (BD Accuri; two lasers) with Special Emphasis on Compensation. Basics on Flow Cytometer Settings - Voltages, Gating, Dump Channels, Exclusion, and Compensation.

b.   Miltenyi MACSQuant Analyzer 10

Advanced Flow Cytometry Laboratory

Analyzing human whole blood and human serum. This rotation includes: Automatic instrument calibration, automatic 8 color compensation, acquisition of whole blood with no RBC lysis, automatic gating/analysis of 8 color immunophenotyping panel, introduction to cytokine multiplex assay, acquisition of cytokine multiplex assay, automatic gating/analysis of cytokine multiplex assay, and automatic shutdown of instrument. In addition to immunophenotyping and multiplex assay, rare cell enrichment and cell counting will be discussed.

c.   Bio-Rad S3e Flow Sorter

Emphasis on Sorting Conditions (parameters, factors, strategies) as well as the Specific Challenges Related to Cell Preparation, Efficiency, Recovery, and Alternate Techniques for Cell Population Identification.

d.   Sony SH800 Flow Sorter

Single Cell Deposition

In Silico Software Training

a.   Introduction to FlowJo Cytometry Analysis Software

b.   Introduction to Cyflogic Cytometry Analysis Software; Flow Cytometry - Acquisition/Trouble Shooting/ Software

c.   Introduction to FlowLogic Cytometry Analysis Software

d.   Introduction to FCS Express Cytometry Analysis Software

Elke Bergmann-Leitner, Ph.D.

Dr. Elke Bergmann-Leitner is the Chief of the Flow Cytometry Center (Malaria Vaccine Branch) at the Walter Reed Army Institute of Research in Silver Spring, MD. She is an adjunct Research Associate Professor of Medicine at the Uniformed Services University of Health Sciences and has been teaching flow cytometry for 25 years. Currently, her main research focus is the development of readout methods capable of identifying immune correlates of protection against infectious diseases.

WLeitnerWolfgang Leitner, Ph.D.

Dr. Leitner is the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH/NIAID). He received his flow cytometry training at the University of Salzburg where he obtained a MSc in biochemistry and a PhD in Immunology. He has been teaching flow cytometry for 25 years. Before joining NIAID, he worked on melanoma and lymphoma vaccines at NCI/NIH, and at WRAIR as a vaccine consultant.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876