August 24-27, 2020

Tuesday - Friday


1 Hour Lunch Break


Workshop Fee

This four day live remote program is ideal for those who are looking for an in-depth introduction to Flow Cytometry.  The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.  Attendees will have laboratory demonstration, protocol reviews and discussion on three different flow platforms (Becton Dickinson, Miltenyi and Bio-Rad) as well as exposure to four software analysis programs.

Flow Cytometry data analysis software:   FlowJo   I   FlowLogic    I    Cyflogic   I  FCS Express 6

remote learning
Real-Time Interactive Lecture, Laboratory Protocol and Discussion
Team taught by active researchers
Reference Materials Included
Lectures, Lab Protocols, Reference Materials included
Space limited to 24 participants
Save Money and Time
No Travel, Convenient and Cost Effective

Course Director

Elke Bergmann-Leitner, Ph.D.
Chief, Flow Cytometry Center

Basic Analytical Techniques, Principles, and Trouble Shooting; Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation); Phenotyping and Analyzing Cell Populations by Flow Cytometry – How to present and interpret flow cytometric data; Introduction to acquisition and analysis Software; Purification and Labeling of Antibodies for Flow Cytometric and Other Ap­plications; Flow Sorting: Critical Experimental Issues and Instrument Setup; Parameters; Functional Flow Cytometric Assays;

Laboratory Demonstrations, Protocols & Discussion:
Preparation of Samples for Multicolor Analysis Flow Sorting; Multicolor Analysis, Compensation and Flow Sorting; Hands-on Flow Cytometry Exercises Using Miltenyi MACSQuant Analyzer; Bio-Rad S3e flow sorter; BD Accuri C6;  Software training: Introduction to FlowJo Cytometry Analysis Software; Introduction to Cyflogic Cytometry Analysis Software; Flow Cytometry - Acquisition/Trouble Shooting/ Software; Introduction to FCS Express Cytometry Analysis Software

Attendees attend all lectures and labs listed. 

Elke Bergmann-Leitner, Ph.D.

Dr. Elke Bergmann-Leitner is the Chief of the Flow Cytometry Center (Malaria Vaccine Branch) at the Walter Reed Army Institute of Research in Silver Spring, MD. She is an adjunct Research Associate Professor of Medicine at the Uniformed Services University of Health Sciences and has been teaching flow cytometry for 25 years. Currently, her main research focus is the development of readout methods capable of identifying immune correlates of protection against infectious diseases.

WLeitnerWolfgang Leitner, Ph.D.

Dr. Leitner is the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH/NIAID). He received his flow cytometry training at the University of Salzburg where he obtained a MSc in biochemistry and a PhD in Immunology. He has been teaching flow cytometry for 25 years. Before joining NIAID, he worked on melanoma and lymphoma vaccines at NCI/NIH, and at WRAIR as a vaccine consultant.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876