This two day live remote program is ideal for basic research and translational biology scientists who work with antibodies on a regular basis. This program which focuses on the types of artifacts associated with antibodies and introduce them to a variety of validation methods that can be implemented in research and academic laboratories.
Antibodies, and in particular monoclonal antibodies, are the single most important laboratory reagent, but are now also recognized as a main contributor to the reproducibility crisis that wastes research funding, undermines trust in scientific research, and jeopardizes scientific careers. This workshop brings to the participants’ attention the various factors that can compromise the validity of data produced with antibodies.
When it comes to verifying the integrity and identity of laboratory reagent, the burden of proof is increasingly being laid on researchers, and thus, the end-user of the reagents. The validation or authentication of antibodies can be performed with techniques and equipment readily available in many laboratories. Therefore, this workshop is recommended for anyone who relies on antibodies in their research.
Real-Time Interactive Lecture, Laboratory Protocol and Discussion
Team taught by active researchers
Lectures, Lab Protocols, Reference Materials included
Space limited to 24 participants
No Travel, Convenient and Cost Effective
" I thoroughly enjoyed this comprehensive and practical presentation of Flow Cytometry – Went back to my home lab and hit the ground running. Thanks for the top-quality training, Bio-Trac!"
FDA NCTR DSB BAMB
" This was a great course. The instructors were very knowledgable & helpful. Without introducing any bias towards particular commercial vendors, the course managed to provide a well balanced flavor for what is out there in terms of options for flow machines and software. The hands on workshops were also very helpful with truly individualized supervision during the classes."
University of Pennsylvania
" Bio-Trac courses are the sure way for one to jump start on a technique’s knowledge and applications base directly from experts and practitioners in the field. They surely give you insights that are not available elsewhere but from years of experiences. knowledge doesn’t stop when a training ends but rather a beginning of one’s development."
Neil H Tan Gana
Ateneo de Manila University, Philippines
"As always, Bio-Trac not only met my expectations, but exceeded them! I will absolutely be taking more classes with BioTrac - their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle."
Assistant Professor, The Ohio State University
"Excellent workshop provided basic and correct gating strategy and respective controls for the specific experiments. Very helpful and whole team was very supportive and explained all my queries at length"
Johns Hopkins Medical Campus (ACCM)
Wolfgang Leitner, PhD
- Reagent authentication: why now? Which reagents have the most “issues”?
- Brief intro to producing antibodies – what can go wrong?
- Antibody batch-to-batch variations and QC
- Problems introduced through antibody labeling
- Problems introduced by antibody purification
- Verifying antibody identity: suitable methods already available in most immunology labs
- Proper maintenance of hybridoma cells, proper storage of antibodies
Hands-on Lab Exercises:
- Antibody Validation by Immunoprecipitation/SDS-PAGE/Western Blotting
- Antibody Validation by Flow Cytometry
- Validating Antibody Avidity with Biosensors
Wolfgang Leitner, PhD, Course Director
Dr. Leitner is the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH/NIAID). He received his flow cytometry training at the University of Salzburg where he obtained a MSc in biochemistry and a PhD in Immunology. He has been teaching flow cytometry for 25 years. Before joining NIAID, he worked on melanoma and lymphoma vaccines at NCI/NIH, and at WRAIR as a vaccine consultant.