This comprehensive lecture/laboratory course is ideal for those bench and research scientists who have a fundamental background in PCR and are looking to gain a thorough understanding of and hands-on exposure to the Real Time, Quantitative and Digital PCR processes.
Lecture and Hands-on Interactive Training
Team taught by active researchers
Comprehensive binder containing workshop material
Space limited to 24 participants
Registration Fee: $1,095
"As always, Bio-Trac not only met my expectations, but exceeded them! I will absolutely be taking more classes with BioTrac - their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle."
Jennie Rowell PhD, RN, Assistant Professor, The Ohio State University
"This is a very well organized program. Lectures always convey the most advanced knowledge and technologies. Hands-on practice is also very helpful. Highly recommend to colleagues in or out of NIH community."
Guangpu Shi, M.D, Research Fellow, NEI/NIH
iPSC: Reprogramming, Differentiation and Gene Editing with CRISPR 8/16
"Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days."
Richard Barrett, PHD Student, University of Central Florida
"It was my first experience with professional development workshop in the USA. I liked it a lot. It was well organized, flexible and friendly. It helped to put in order my preceding knowledge and gain more."
Larisa Ryzhara, MD, PhD, Staff Scientist II, Maine Medical Center Research Institute
Intro to real-time PCR; Short History of real-time PCR; Sample Processing (DNA/RNA Extraction and Purification for Real-time PCR); Probe Chemistries and Real-time PCR Instrumentation; Assay Development; Design, function and use of TaqMan & TaqMan-MGB probes; Design, function and use of SimpleProbes and HybProbes; Target selection for real-time PCR; Optimization of TaqMan, TaqMan-MGB, HybProbe and SimpleProbes; Advanced testing (validation) of optimized assays; Medical applications of real-time PCR; Assessing, monitoring, and testing PCR inhibition; Contamination; Quality control; Array qPCR; Multiplex Real-Time PCR; Quantitative Real-Time PCR; Digitial PCR (dPCR);
Sample Processing Lab: DNA extraction by manual and automated meth- ods; RNA extraction and Sybr Green Real-Time RT-PCR; 3. Real-time PCR lab (set up and run DNA and real-time PCR assays on the LC 480 w/ LC 480 Control Kits & Extracted DNA); Analysis of RT-PCR Data; Use of Real-time + PCR Assay Software; Array qPCR (RT and Array Set-up); Analysis of Array qPCR Data; Quantitative Real-Time PCR Lab Set-up; Assemble dPCR Reactions and Initiate Run
- Intro to real-time PCR; Short History of real-time PCR
- Sample Processing
- Sample Processing Lab: DNA extraction by manual and automated meth- ods; RNA extraction and Sybr Green Real-Time RT-PCR
- Probe Chemistries and Real-time PCR Instrumentation
- Assay Development (Part I); Design, function and use of TaqMan & TaqMan-MGB probes; Design, function and use of SimpleProbes and HybProbes; Target selection for real-time PCR
- Real-time PCR lab (set up and run DNA and real-time PCR assays on the LC 480 w/ LC 480 Control Kits & Extracted DNA)
- Analysis of RT-PCR Data
- Use of Real-time + PCR Assay Software
- Assay Development (Part 2); Optimization of TaqMan, TaqMan-MGB, HybProbe and SimpleProbes; Advanced testing (validation) of optimized assays; Medical applications of real-time PCR; Assessing, monitoring, and testing PCR inhibition; Contamination; Quality contro
- Array qPCR
- Multiplex Real-Time PCR
- Array qPCR (RT and Array Set-up) – Runs overnight
- Quantitative Real-Time PCR
- Analysis of Array qPCR Data
- Quantitative Real-Time PCR Lab Set-up
- Discussion of Quantitative Real-Time PCR Results
- Digitial PCR (dPCR)
- Wrap-up Lecture
- Assemble dPCR Reactions and Initiate Run
- d-PCR Data Analysis
Dr. David A. Kulesh is a research microbiologist who has been working in the Molecular Diagnostics Department at US Army Medical Research Institute of Infectious Diseases (USAMRIID) for over 16 years. Dr. Kulesh has a PhD and Masters in Biology from The Catholic Uniersity of America (CUA) and a BS from Villanova University. Prior to his employment as a US Army civilian, Dr. Kulesh was a LtCol in the US Air Force stationed at both The Armed Forces Institute of Pathology (AFIP) and USAMRIID. While at AFIP, Dr Kulesh was invloved with studying the effects of microgravity on the differentiation of muscle cells by sending tissue cultures aboard 5 Space Shuttle missions. At USAMRIID, Dr. Kulesh has been responsible for the development of multiple real-time qPCR assays used in the detection of both biothreat bacteria and viruses ranging from anthrax to smallpox to Ebola. His Ebola real-time qPCR assays were used by the US Army during the 2015 West Africa outbreak. Dr. Kulesh has been invlolved in the molecular workshops since he was a graduate student at CUA over 30 years ago.