This five day workshop is ideal for bench and research scientists who are looking for a solid foundation in RNA-Seq, from application, experimental design and sample preparation to post sequencing data processing analysis using latest bioinformatics approaches.
Lecture and Hands-on Interactive Training
Team taught by active researchers
Comprehensive binder containing workshop material
Space limited to 24 participants
Registration Fee: $1,095
"As always, Bio-Trac not only met my expectations, but exceeded them! I will absolutely be taking more classes with BioTrac - their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle."Jennie Rowell PhD, RN, Assistant Professor, The Ohio State University CRISPR 7/16
"This is a very well organized program. Lectures always convey the most advanced knowledge and technologies. Hands-on practice is also very helpful. Highly recommend to colleagues in or out of NIH community."
Guangpu Shi, M.D, Research Fellow, NEI/NIH
iPSC: Reprogramming, Differentiation and Gene Editing with CRISPR 8/16
"Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days."
Richard Barrett, PHD Student, University of Central Florida
"It was my first experience with professional development workshop in the USA. I liked it a lot. It was well organized, flexible and friendly. It helped to put in order my preceding knowledge and gain more."
Larisa Ryzhara, MD, PhD, Staff Scientist II, Maine Medical Center Research Institute
This workshop is a unique mix of hands-on wet bench and electronic laboratory experiences together with a series of lectures spanning introduction to the concepts of RNA sequencing and the analysis of the data generated to the applications of these approaches to understand important biological and/or biomedical processes and problems. At the end of the program, attendees will have had hands-on experience with the more challenging aspects of manual preparation of an optimized RNA library, analysis of RNA sequences from control and Alzheimer’s Disease brain samples, and knowledge of the application of the most current programs to the analysis of these data.
- Introduction to NGS and Course Overview
- From Next Generation Sequencing to RNA Seq
- Introduction to Downstream Analysis
- Important Considerations for Downstream Analysis
- Practical Aspects of RNA-Seq
- RNA Library Preparation Overview; MiSeq Run Monitoring and Quality Control
- RNA-Seq Gene Expression Data Analysis Pipeline: Methods, Tools and Issues
- MiSeq Data Analysis – From Analysis to Networks
- 16S Metagenomics Analysis; Bioconductor and RNA-Seq Data Analysis
- RNA-seq of the small RNAs
- RNA-seq data analysis in the context of biological networks
- The Interplay between Environmental exposures and metabolic disorders – integrating transcriptional changes and pathway analysis
- Effect of salt loading on RNA-seq analysis of rat supraoptic nucleus transcriptome
- Basics of statistical analysis of RNA-seq data
- Sample Preparation and Run Set-up
- Demo/Hands-on Library Prep Magnetic Beads Separation
- Illumina MiSeq: Sequencing of samples from AD brains plus opportunity to sequence RNA from one selected attendee
- Run assessment
- PC Based: Using BaseSpace to Analyze RNA-Seq Data
- Post-RNA Seq Analysis
- Pathway Analysis
- Bioconductor and RNA-Seq Data Analysis
- RNA-Seq Data Analysis in the Context of Biological Networks
Dr. Frank Castora is a Professor of Biochemistry in the Department of Physiological Sciences at the Eastern Virginia Medical School. Dr. Castora has been involved with teaching in or directing a variety of Bio-Trac training programs at the National Institutes of Health as well as other institutions nationally for over thirty years, including workshops in mitochondrial biogenesis and pathology, recombinant DNA technology, PCR, microarray analysis, and RNA-seq. Currently his research interests include mitochondrial DNA mutations and gene expression in AD as well as mitochondrial function in human reproduction and fertility