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Bio-Trac®

Flow Cytometry

  • Scope: Flow Cytometry

Flow Cytometry: Principles and Methods

This lecture/laboratory workshop will cover various applications of flow cytometry in basic research. Selected lecture topics will provide a broad background about the functions of a flow cytometer; choice of fluorochromes; data analysis and presentation; technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis.

Lecture and Laboratory Topics:
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; Blocking with Antibodies); Software; Laser Scanning Cytometry; Proliferation using Bio-Tracers

Please contact us for more information on a customized training workshop delivered on-site, on budget and on your schedule.