BIO-TRAC
FAES/NIH
Building 60
Room 237
1 Cloister Court
Bethesda, MD
20814-1460
301-496-8290
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TRAC 33: Clinical Proteomics and Biomarker Discovery
The emerging field of Clinical Proteomics refers to the application of proteomic technologies to investigate protein expression differences in clinically obtained biological samples. A consequence of these approaches has been the renewed interest in identifying new biomarkers which may useful for the diagnosis of disease or monitoring of patient response to therapy. Researchers involved in Clinical Proteomics face numerous methodological, analytical, and statistical challenges that need to be addressed in order for successful completion of projects. Additionally, large scale biomarker discovery efforts include the coordination of many different disciplines, such as biochemistry, proteomics and mass spectrometry, clinical chemistry and bioinformatics. In this course, students will be exposed to many of the challenging aspects of biomarker discovery projects, as well as to the numerous analytical platforms that may be employed.
Topics: Study Design and Sample Collection/Storage of Clinical Samples; Sample Handling and Preparation (Chromatographic methods to reduce the complexity of the sample, such as ion-exchange chromatography, immunoaffinity depletion); Quality Control and Reproducibility in Measurements; Analytical Tools for the measurement of quantitative difference between clinical samples: 2D gel electrophoresis, including DIGE; MALDI/SELDI-TOF-MS for Serum Protein Profiling, Multiplex Protein/Cytokine Arrays, Differential isotopic labeling, such as ICAT, SILAC Quantitative Data Analysis of 2D and Mass Spec Data Sets; Multivariate Statistical Models; MS Protein Patters vs. Protein Panels for Diagnostic Determination |
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| ONE WEEK COURSE |
| TRAC 33 |
| June 20 - 24, 2011 |
| REGISTER-SU |
| TIME |
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9:00 - 5:00 p.m.
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| 35 Contact Hours |
| FEE |
| $850 |
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