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TRAC 29: Laser Microdissection: Methods for Microgenomic Analysis
Laser Microdissection systems allows for the procurement of specific populations of cells from tissue and cytology and live cell culture samples containing heterogeneous populations of cells. The specificity of analyses are therefore much more representative of the disease process being studied. This approach to microdissection ensures that biological molecules, such as RNA and DNA and proteins, remain undamaged during the microdissection process. Downstream molecular analysis of these molecules produces accurate and assured results that have led to over 2,000 peer-reviewed publications by independent researchers.
In this four-day training program, participants learn to prepare tissue specimens for microdissection, then select and acquire homogenous cell populations using the mmi-CellCut, Leica LMD, Arcturus XT, Veritas and PALM microdissection systems. Instruction emphasizes operation of these LM systems, appropriate tissue handling and sample preparation for subsequent DNA, RNA or protein analysis, and methods for proper molecular extraction. Lecture and detailed instructions to prepare samples for several downstream molecular analyses are presented.
Topics: Overview of Laser Microdissection Technology (History, Theory, Applications); Tissue Sample and Slide preparation; Project set up and QC of microdissected samples, Genomic and Epigenetic Analysis using PCR and microarray-based platforms, mRNA analysis including quantitative RT-PCR gene expression analysis and microarrays from microdissected tissue samples, microRNA analysis from microdissected samples, and proteomic analysis from frozen and formalin-fixed, paraffin-embedded microdissected samples using Mass Spec. The lectures also include examples of scientific and clinical applications of LM-based studies. The hands on sessions include practice on Arcturus XT, PALM, Leica and mmi-CellCut platforms.
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