October 11-14, 2016

Tuesday - Friday

Germantown, MD

The Bioscience Education Center

9:00am-5:00pm

1 Hour Lunch Break

This four day program is ideal for those who are looking for an in-depth, hands-on introduction to Flow Cytometry.  The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.  Attendees will have hands-on experience on three different flow platforms (Becton Dickinson, Miltenyi and Bio-Rad) as well as exposure to three software analysis programs.

Comprehensive binder containing workshop material
Space limited to 18 participants
Registration Fee: $995

"I thoroughly enjoyed this comprehensive and practical presentation of Flow Cytometry – Went back to my home lab and hit the ground running. Thanks for the top-quality training, Bio-Trac!"

Gwenn Merry
FDA NCTR DSB BAMB (FACS 7/16)

Course Outline

Tuesday, October 11, 2016 
9:00-12:00
Dr. Bergmann -Leitner, WRAIR
Lecture: Introductory FACS lecture; Overview of Flow Cytometric Applications; Basic Analytical Techniques, Principles, and Trouble Shooting
12:00-1:00Lunch
1:00-2:30
Dr. Bergmann -Leitner, WRAIR
Lecture: Principles of Cell Staining, Choosing Appropriate Controls, Instrument Setup
2:45-4:15
Dr. Bergmann-Leitner, WRAIR, Dr. Melake, MC
Laboratory: Preparation of Samples for Multicolor Analysis Flow Sorting
Wednesday, October 12, 2016 
9:00-10:30
Dr. Bergmann -Leitner, WRAIR
Lecture: Multicolor Flow Cytometry and Panel Development
10:30-12:00
Dr. Bergmann-Leitner, WRAIR
Lecture: Phenotyping and Analyzing Cell Populations by Flow Cytometry - How to Read Flow Data
12:00-1:00Lunch
1:00-3:00Laboratory Rotation I
3:15-5:15Laboratory Rotation II

Hands-on Flow Cytometry Laboratory Rotations

Participants will rotate between stations in small groups of six. Groups will visit each station for two hours.

Thursday, October 13, 2016 
9:00-10:30
Dr. Leitner, NIAID/NIH
Lecture: Purification and Labeling of Antibodies for Flow Cytometric and other Applications
10:30-12:00
Dr. Leitner, NIAID/NIH
Lecture: Flow Sorting: Instrument Setup; Parameters
12:00-1:00Lunch
1:00-3:00Laboratory Rotation III
3:15-5:15
Dr. Leitner, NIAID/NIH
Lecture: Functional Assays for the Flow Cytometry, Part 1
Friday, October 14, 2016 
8:00-9:30
Dr. Leitner, NIAID/NIH
Lecture: Functional Assays for the Flow Cytometer, Part 2
9:45 -11:45
Dr. Leitner, NIAID/NIH
Data Workshop: Hands-on Data Analysis; Samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers
11:45-12:45Lunch
12:45-2:45
Ms. Farooq, WRAIR
Software Training: Introduction to FlowJo Cytometry Analysis Software
2:55-3:55
Dr. Beck, Miltenyi Biotec, Inc
Software Training: Introduction to FlowLogic Cytometry Analysis Software
4:00-5:00
Dr. Bergmann-Leitner, Dr. Leitner
Small Group Sessions: Flow Cytometry - Lecture or Laboratory (your choice)
Acquisition/Trouble Shooting/Software
20 minute time slot rotations

FACs Data Analysis Software

COURSE DIRECTOR

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Elke Bergmann-Leitner, Ph.D. 
Chief, Flow Cytometry Center
USMMRP/WRAIR

Dr. Elke Bergmann-Leitner is the Chief of the Flow Cytometry Center (Malaria Vaccine Branch) at the Walter Reed Army Institute of Research in Silver Spring, MD. She is an adjunct Research Associate Professor of Medicine at the Uniformed Services University of Health Sciences and has been teaching flow cytometry for 25 years. Currently, her main research focus is the development of readout methods capable of identifying immune correlates of protection against infectious diseases.

INSTRUCTOR

Wolfgang Leitner, Ph.D.
Chief of the Innate Immunity Section
NIAID/NIH, USA

Dr. Wolfgang Leitner the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH). He has been teaching flow cytometry for 25 years. He previously worked at the National Cancer Institute where he developed various flow cytometric protocols for the evaluation of experimental cancer vaccines.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876

Flow Cytometry: Principles, Methods & Applications

In-depth, hands-on introduction to Flow Cytometry. Don't miss out.