March 7-10, 2016

Tuesday - Friday

Germantown, MD

The Bioscience Education Center

9:00am-5:00pm

1 Hour Lunch Break

This four day program is ideal for those who are looking for an in-depth, hands-on introduction to Flow Cytometry.  The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.  Attendees will have hands-on experience on three different flow platforms (Becton Dickinson, Miltenyi and Bio-Rad) as well as exposure to three software analysis programs.

FACs platforms:   BD Accuri C6   I   Miltenyi Biotec MACSQuant Analyzer   I   Bio-Rad S3e
FACs data analysis software:   FlowJo   I   FlowLogic    I    Cyflogic

NOTE: This course will be offered a second time August 22-25, 2017.  Preregistration available.

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Lecture and Hands-on Interactive Training
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Team taught by active researchers
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Comprehensive binder containing workshop material
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Space limited to 18 participants
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Registration Fee: $995

Course Director

Elke Bergmann-Leitner, Ph.D.
Chief, Flow Cytometry Center
USMMRP/WRAIR

Lectures
Basic Analytical Techniques, Principles, and Trouble Shooting; Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation); Phenotyping and Analyzing Cell Populations by Flow Cytometry – How to present and interpret flow cytometric data; Introduction to acquisition and analysis Software; Purification and Labeling of Antibodies for Flow Cytometric and Other Ap­plications; Flow Sorting: Critical Experimental Issues and Instrument Setup; Parameters; Functional Flow Cytometric Assays;

Laboratory:
Preparation of Samples for Multicolor Analysis Flow Sorting; Multicolor Analysis, Compensation and Flow Sorting; Hands-on Flow Cytometry Exercises Using Miltenyi MACSQuant Analyzer; Bio-Rad S3e flow sorter; BD Accuri C6;  Software training: Introduction to FlowJo Cytometry Analysis Software; Introduction to Cyflogic Cytometry Analysis Software; Flow Cytometry - Acquisition/Trouble Shooting/ Software

Attendees attend all lectures and labs listed. 

Flow Cytometric Applications

  • Lecture: Basic Analytical Techniques, Principles, and Trouble Shooting
  • Lecture: Principles of Cell Staining, Choosing the Right Controls, Instrument Setup
  • Laboratory: Preparation of Samples for Multicolor Analysis Flow Sorting

Full Class Lectures

  • Multicolor Flow Cytometry and Panel Development
  • Phenotyping and Analyzing Cell Populations by Flow Cytometry - How to Read Flow Data

Small Learning Groups

  • Refer to modules tab for descriptions

Day 2 & 3: Small Learning Groups

MODULE 1 

  • Hands-on Laboratory : Multicolor Analysis, Compensation and Flow Sorting
  • Lecture: Purification and Labeling of Antibodies for flow cytometric and other applications

MODULE 2

  • Hands-on Laboratory : MACSQuant Analyzer
  • Lecture: Flow Sorting: Instrument Setup; Parameters

Lecture

  • Analysis of cell cycle, cellular proliferation by flow cytometry

Laboratories

  • Data Workshop; Hands-on Data Analysis; Samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers
  • Software training: Introduction to FlowJo Cytometry Analysis Software; Introduction to FlowLogic Cytometry Analysis Software; Introduction to Cyflogic Cytometry Analysis Software

Small Group Sessions

  • Flow Cytometry - Acquisition/Trouble Shooting/Software
  • 20 minute time slot rotations - student choice

Elke Bergmann-Leitner, Ph.D.

Dr. Elke Bergmann-Leitner is the Chief of the Flow Cytometry Center (Malaria Vaccine Branch) at the Walter Reed Army Institute of Research in Silver Spring, MD. She is an adjunct Research Associate Professor of Medicine at the Uniformed Services University of Health Sciences and has been teaching flow cytometry for 25 years. Currently, her main research focus is the development of readout methods capable of identifying immune correlates of protection against infectious diseases.

WLeitnerWolfgang Leitner, Ph.D.

Dr. Wolfgang Leitner the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH). He has been teaching flow cytometry for 25 years. He previously worked at the National Cancer Institute where he developed various flow cytometric protocols for the evaluation of experimental cancer vaccines.

The Bioscience Education Center

Montgomery College
20200 Observation Drive
Germantown, MD 20876