This four day program is ideal for those who are looking for an in-depth, hands-on introduction to Flow Cytometry. The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design. Attendees will have hands-on experience on three different flow platforms (Becton Dickinson, Miltenyi and Bio-Rad) as well as exposure to three software analysis programs.
Lecture and Hands-on Interactive Training
Team taught by active researchers
Comprehensive binder containing workshop material
Space limited to 18 participants
Registration Fee: $995
" I thoroughly enjoyed this comprehensive and practical presentation of Flow Cytometry – Went back to my home lab and hit the ground running. Thanks for the top-quality training, Bio-Trac!"
Gwenn Merry, FDA NCTR DSB BAMB
"This is a very well organized program. Lectures always convey the most advanced knowledge and technologies. Hands-on practice is also very helpful. Highly recommend to colleagues in or out of NIH community."
Guangpu Shi, M.D, Research Fellow, NEI/NIH
iPSC: Reprogramming, Differentiation and Gene Editing with CRISPR 8/16
"As always, Bio-Trac not only met my expectations, but exceeded them! I will absolutely be taking more classes with BioTrac - their classes should be required for all graduates entering the field of science. Easy to understand & follow, yet the classes get to the core of the discussed principle."
Jennie Rowell PhD, RN, Assistant Professor, The Ohio State University
"Great investment. Took material that would have taken months to compile and perfect on my own, and packed it into 3 days."
Richard Barrett, PHD Student, University of Central Florida
Basic Analytical Techniques, Principles, and Trouble Shooting; Principles of Cell Staining, Choosing the Right Experimental Controls, Instrument Setup (baseline and compensation); Phenotyping and Analyzing Cell Populations by Flow Cytometry – How to present and interpret flow cytometric data; Introduction to acquisition and analysis Software; Purification and Labeling of Antibodies for Flow Cytometric and Other Applications; Flow Sorting: Critical Experimental Issues and Instrument Setup; Parameters; Functional Flow Cytometric Assays;
Preparation of Samples for Multicolor Analysis Flow Sorting; Multicolor Analysis, Compensation and Flow Sorting; Hands-on Flow Cytometry Exercises Using Miltenyi MACSQuant Analyzer; Bio-Rad S3e flow sorter; BD Accuri C6; Software training: Introduction to FlowJo Cytometry Analysis Software; Introduction to Cyflogic Cytometry Analysis Software; Flow Cytometry - Acquisition/Trouble Shooting/ Software
Attendees attend all lectures and labs listed.
Flow Cytometric Applications
- Lecture: Basic Analytical Techniques, Principles, and Trouble Shooting
- Lecture: Principles of Cell Staining, Choosing the Right Controls, Instrument Setup
- Laboratory: Preparation of Samples for Multicolor Analysis Flow Sorting
Full Class Lectures
- Multicolor Flow Cytometry and Panel Development
- Phenotyping and Analyzing Cell Populations by Flow Cytometry - How to Read Flow Data
Small Learning Groups
- Refer to modules tab for descriptions
Day 2 & 3: Small Learning Groups
- Hands-on Laboratory : Multicolor Analysis, Compensation and Flow Sorting
- Lecture: Purification and Labeling of Antibodies for flow cytometric and other applications
- Hands-on Laboratory : MACSQuant Analyzer
- Lecture: Flow Sorting: Instrument Setup; Parameters
- Analysis of cell cycle, cellular proliferation by flow cytometry
- Data Workshop; Hands-on Data Analysis; Samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers
- Software training: Introduction to FlowJo Cytometry Analysis Software; Introduction to FlowLogic Cytometry Analysis Software; Introduction to Cyflogic Cytometry Analysis Software
Small Group Sessions
- Flow Cytometry - Acquisition/Trouble Shooting/Software
- 20 minute time slot rotations - student choice
Elke Bergmann-Leitner, Ph.D.
Dr. Elke Bergmann-Leitner is the Chief of the Flow Cytometry Center (Malaria Vaccine Branch) at the Walter Reed Army Institute of Research in Silver Spring, MD. She is an adjunct Research Associate Professor of Medicine at the Uniformed Services University of Health Sciences and has been teaching flow cytometry for 25 years. Currently, her main research focus is the development of readout methods capable of identifying immune correlates of protection against infectious diseases.
Wolfgang Leitner, Ph.D.
Dr. Wolfgang Leitner the Chief of the Innate Immunity Section at the National Institute of Allergy and Infectious Diseases (NIH). He has been teaching flow cytometry for 25 years. He previously worked at the National Cancer Institute where he developed various flow cytometric protocols for the evaluation of experimental cancer vaccines.